A water soluble β-glucan (PS) with an average molecular weight ∼1.95 × 10(5)Da was isolated from the alkaline extract of ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr. and found to consist of terminal, (1 → 3)-, (1 → 6)-, and (1 → 3,6)-linked β-D-glucopyranosyl moieties in a ratio of nearly 1:2:2:1. The structure of this PS was elucidated on the basis of total hydrolysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR experiments. On the basis of these experiments, the repeating unit of the PS was found to contain a backbone of three (1 → 6)-β-D-glucopyranosyl residues, one of which was branched at O-3 position with the side chain consisting of two (1 → 3)-β-D-glucopyranosyl and a terminal β-D-glucopyranosyl residue. This PS showed in vitro macrophage activation by NO production as well as splenocytes and thymocytes proliferation. Moreover, it also exhibited potent antioxidant activities.
Keywords: Antioxidant activities; Immunostimulation; NMR studies; Russula albonigra (Krombh.) Fr.; β-Glucan.
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