Abstract
Cancer-associated human papillomaviruses (HPVs) express E6 oncoproteins that target the degradation of p53 and have a carboxy-terminal PDZ ligand that is required for stable episomal maintenance of the HPV genome. We find that the E6 PDZ ligand can be deleted and the HPV genome stably maintained if cellular p53 is inactivated. This indicates that the E6-PDZ interaction promotes HPV genome maintenance at least in part by neutralization of an activity that can arise from residual undegraded p53.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line
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DNA Replication
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Gene Expression Regulation, Viral
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Genome, Viral*
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Humans
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Oncogene Proteins, Viral / chemistry
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / metabolism*
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PDZ Domains*
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Papillomaviridae / genetics*
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Papillomaviridae / metabolism*
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Protein Binding
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RNA Interference
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
Substances
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E6 protein, Human papillomavirus type 16
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Oncogene Proteins, Viral
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Repressor Proteins
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Tumor Suppressor Protein p53