Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography

Food Chem. 2014:152:386-90. doi: 10.1016/j.foodchem.2013.11.080. Epub 2013 Nov 22.

Abstract

Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and (1)H and (13)C nuclear magnetic resonance (NMR) spectra.

Keywords: Blue honeysuckle fruits; Cyanidin 3-glucoside; High-speed counter-current chromatography (HSCCC).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / analysis
  • Anthocyanins / isolation & purification*
  • Countercurrent Distribution
  • Fruit / chemistry*
  • Glucosides / analysis
  • Glucosides / isolation & purification*
  • Lonicera / chemistry*
  • Plant Extracts / analysis
  • Plant Extracts / isolation & purification*

Substances

  • Anthocyanins
  • Glucosides
  • Plant Extracts
  • cyanidin-3-O-beta-glucopyranoside