Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey

Biosens Bioelectron. 2014 Jul 15:57:192-8. doi: 10.1016/j.bios.2014.02.032. Epub 2014 Feb 19.

Abstract

Tetracycline (TC) is widely used for prevention and control of animal diseases for its broad spectrum antimicrobial activity and low cost, but its abuse can seriously affect human health and may result in trade loss. Thus there is an imperative need to develop high-performing analytical technique for TC detection. In this study, we developed a biosensor based on an indirect competitive enzyme-linked aptamer assay (ic-ELAA). A 76mer single-stranded DNA (ssDNA) aptamer, selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), was applied for the recognition and detection of TC in honey. The limit of detection was 9.6×10(-3) ng/mL with a linear working range from 0.01 to 100 ng/mL toward TC in honey, and a mean recovery rate of 93.23% in TC-spiked honey was obtained. This aptasensor can be applied to detect TC residue in food with high sensitivity and simplicity, and it is prospective to develop useful ELAA Kits for TC determination in food.

Keywords: Aptamer; Aptasensor; Enzyme-linked aptamer assay; Honey analysis; Tetracycline.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / analysis*
  • Aptamers, Nucleotide / chemistry*
  • Biosensing Techniques / methods
  • DNA, Single-Stranded / chemistry*
  • Food Contamination / analysis*
  • Honey / analysis*
  • Limit of Detection
  • SELEX Aptamer Technique
  • Tetracycline / analysis*

Substances

  • Anti-Bacterial Agents
  • Aptamers, Nucleotide
  • DNA, Single-Stranded
  • Tetracycline