Simultaneous detection of four garlic viruses by multiplex reverse transcription PCR and their distribution in Indian garlic accessions

J Virol Methods. 2014 Jun:202:34-8. doi: 10.1016/j.jviromet.2014.02.019. Epub 2014 Mar 2.

Abstract

Indian garlic is infected with Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garlic common latent virus (GarCLV) and allexiviruses. Identity and distribution of garlic viruses in various garlic accessions from different geographical regions of India were investigated. OYDV and allexiviruses were observed in all the garlic accessions, while SLV and GarCLV were observed only in a few accessions. A multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection and identification of OYDV, SLV, GarCLV and Allexivirus infecting garlic accessions in India. This multiplex protocol standardized in this study will be useful in indexing of garlic viruses and production of virus free seed material.

Keywords: Allexivirus; GarCLV; Garlic viruses; Multiplex RT-PCR; OYDV; SLV.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carlavirus / genetics
  • Carlavirus / isolation & purification*
  • Flexiviridae / genetics
  • Flexiviridae / isolation & purification*
  • Garlic / virology*
  • India
  • Multiplex Polymerase Chain Reaction / methods*
  • Multiplex Polymerase Chain Reaction / standards
  • Phylogeography*
  • Potyvirus / genetics
  • Potyvirus / isolation & purification*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / standards