Biocompatible and photostable AIE dots with red emission for in vivo two-photon bioimaging

Dan Wang; Jun Qian; Wei Qin; Anjun Qin; Ben Zhong Tang; Sailing He

doi:10.1038/srep04279

Biocompatible and photostable AIE dots with red emission for in vivo two-photon bioimaging

Sci Rep. 2014 Mar 17:4:4279. doi: 10.1038/srep04279.

Authors

Dan Wang¹, Jun Qian², Wei Qin³, Anjun Qin⁴, Ben Zhong Tang⁵, Sailing He⁶

Affiliations

¹ 1] Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China [2] State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China [3].
² 1] Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China [2].
³ Department of Chemistry, Division of Biomedical Engineering, and Institute for Advanced Study, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
⁴ MoE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
⁵ 1] Department of Chemistry, Division of Biomedical Engineering, and Institute for Advanced Study, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China [2] Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou 510640, China.
⁶ Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China.

Abstract

Bioimaging systems with cytocompatibility, photostability, red fluorescence, and optical nonlinearity are in great demand. Herein we report such a bioimaging system. Integration of tetraphenylethene (T), triphenylamine (T), and fumaronitrile (F) units yielded adduct TTF with aggregation-induced emission (AIE). Nanodots of the AIE fluorogen with efficient red emission were fabricated by encapsulating TTF with phospholipid. The AIE dots enabled three-dimensional dynamic imaging with high resolution in blood vessels of mouse brain under two-photon excitation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines / chemistry
Animals
Blood Vessels / drug effects
Blood Vessels / ultrastructure*
Brain / blood supply
Brain / drug effects
Brain / ultrastructure*
Diagnostic Imaging / methods*
Drug Carriers / chemistry
Drug Compounding
Fluorescent Dyes
Fumarates / chemistry
Mice
Microscopy, Fluorescence, Multiphoton
Photons*
Polyethylenes / chemistry
Quantum Dots* / chemistry
Terphenyl Compounds / chemistry

Substances

Amines
Drug Carriers
Fluorescent Dyes
Fumarates
Polyethylenes
Terphenyl Compounds
fumaronitrile