A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2

Arch Virol. 2014 Sep;159(9):2519-21. doi: 10.1007/s00705-014-2069-4. Epub 2014 Apr 10.

Abstract

Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5' terminus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cluster Analysis
  • Ecuador
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology*
  • RNA Viruses / genetics*
  • RNA Viruses / isolation & purification*
  • RNA, Viral / genetics*
  • Recombination, Genetic*
  • Rosaceae / virology*
  • Sequence Analysis, DNA

Substances

  • RNA, Viral

Associated data

  • GENBANK/KJ007639
  • GENBANK/KJ007640