Objective: To investigate the effects of chlorogenic acid on the viability and HIF-1alpha mRNA expression of PC12 cells exposed to hypoxia.
Methods: PC12 cells were cultured in trigas incubator in order to establish the hypoxic condition. The effects of chlorogenic acid on the cells were evaluated by morphological observation, cell viability and LDH release assays as well as the examination of mRNA expression level of HIF-1alpha.
Results: Chlorogenic acid significantly improved the viability of cells exposed to hypoxia, decreased LDH release, arrested the cell cycle on G1 phase, and increased the gene expression level of HIF-1alpha.
Conclusion: Chlorogenic acid protects PC12 cells from hypoxic damage by improving the expression of HIF-1alpha.