Nine human sparganosis cases in Thailand with molecular identification of causative parasite species

Am J Trop Med Hyg. 2014 Aug;91(2):389-93. doi: 10.4269/ajtmh.14-0178. Epub 2014 May 19.

Abstract

Human sparganosis is one of the neglected diseases but important food-borne parasitic zoonoses. The disease is caused by larvae (spargana) of diphyllobothriidean tapeworm. Here, we describe nine cases of human sparganosis, caused by Spirometra erinaceieuropaei in a hospital in Thailand during 2001-2012. Clinical characteristics, treatment, and outcome of cases were revealed. Diagnosis and identification of causative parasite species was made by histopathological investigations followed by a polymerase chain reaction-based molecular method using formalin-fixed paraffin embedded tissues. The DNA samples were extracted from tissues and a partial fragment of cytochrome c oxidase subunit 1 (cox1) gene was amplified for the detection of parasitic DNA. Infection could be prevented by increasing activities on health communication by responsible public health agencies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • DNA, Helminth / classification
  • DNA, Helminth / genetics*
  • Electron Transport Complex IV / classification
  • Electron Transport Complex IV / genetics*
  • Female
  • Formaldehyde
  • Humans
  • Incidence
  • Male
  • Middle Aged
  • Neglected Diseases*
  • Phylogeny
  • Polymerase Chain Reaction
  • Sparganosis / epidemiology*
  • Sparganosis / parasitology
  • Sparganosis / pathology
  • Sparganosis / surgery
  • Sparganum / classification
  • Sparganum / genetics*
  • Sparganum / isolation & purification
  • Thailand / epidemiology
  • Tissue Embedding

Substances

  • DNA, Helminth
  • Formaldehyde
  • Electron Transport Complex IV