We propose a real-time integral imaging system for light field microscopy systems. To implement a 3D live in-vivo experimental environment for multiple experimentalists, we generate elemental images for an integral imaging system from the captured light field with a light field microscope in real-time. We apply the f-number matching method to generate an elemental image to reconstruct an undistorted 3D image. Our implemented system produces real and orthoscopic 3D images of micro objects in 16 frames per second. We verify the proposed system via experiments using Caenorhabditis elegans.