Regulation of extracellular oxidoreduction potential enhanced (R,R)-2,3-butanediol production by Paenibacillus polymyxa CJX518

Bioresour Technol. 2014 Sep:167:433-40. doi: 10.1016/j.biortech.2014.06.044. Epub 2014 Jun 19.

Abstract

Cellular redox status and oxygen availability influence the product formation. Herein, decreasing agitation speed or adding vitamin C (Vc) achieved the 2,3-BDL yield of 0.40 g g(-1) or 0.39 g g(-1)glucose under batch fermentation, respectively. To our knowledge, this is the highest 2,3-BDL yield reported so far for Paenibacillus polymyxa without adding acetic acid. The NADH/NAD(+) ratio and 2,3-BDL titer could be increased significantly by reducing the agitation speed or adding Vc, indicating that the enhancement of 2,3-BDL is closely associated with the adjustment of NADH/NAD(+) ratio. Especially, Vc addition elevated the 2,3-BDL titer from 43.66 g L(-1) to 71.71 g L(-1) within 54 h under fed-batch fermentation. This is the highest titer of 2,3-BDL so far reported for P. polymyxa from glucose fermentation. This work provides a new strategy to improve 2,3-BDL production and helps us to understand the responses of P. polymyxa to extracellular oxidoreduction potential.

Keywords: (R,R)-2,3-butanediol; NADH/NAD(+) ratio; Paenibacillus polymyxa; Redox regulation; Vitamin C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid / pharmacology
  • Batch Cell Culture Techniques
  • Borohydrides / pharmacology
  • Butylene Glycols / metabolism*
  • Extracellular Space / metabolism*
  • Fermentation / drug effects
  • Intracellular Space / metabolism
  • Molecular Sequence Data
  • NAD / metabolism
  • Oxidation-Reduction / drug effects
  • Oxygen / pharmacology
  • Paenibacillus / drug effects
  • Paenibacillus / metabolism*
  • Temperature

Substances

  • Borohydrides
  • Butylene Glycols
  • NAD
  • 2,3-butylene glycol
  • sodium borohydride
  • Ascorbic Acid
  • Oxygen

Associated data

  • GENBANK/KF991241