Abstract
This study evaluates the influence of novel galacto-oligosaccharides derived from lactulose (GOS-Lu), kojibiose or 4'-galactosyl-kojibiose in hematological parameters of Fe homeostasis using Fe-deficient animals. Liver TfR-2, IL-6, NFκB and PPAR-γ expression (mRNA) were also determined by RT-qPCR analyses, and active hepcidin peptide production and short chain fatty acids by LC coupled to MS/MS or UV detection. Feeding animals with GOS-Lu or kojibiose together with FeCl3 increased hemoglobin (Hb) production (by 17%) and mean Hb concentration into erythrocytes relative to animals administered with FeCl3 alone (14.1% and 19.7%, respectively). Animals administered with prebiotics showed decreased plasmatic hepcidin levels, contributing to a higher intestinal absorption of the micronutrient. These data indicate that concurrent administration of these potentially prebiotic oligosaccharides together with a supplement of Fe ameliorates inflammation-mediated perturbations in the liver, according to the particular structure of the prebiotic compound, and result an attractive strategy to improve Fe absorption.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anemia, Iron-Deficiency / drug therapy*
-
Animals
-
Chlorides / administration & dosage
-
Chlorides / pharmacokinetics
-
Dietary Supplements
-
Disease Models, Animal
-
Fatty Acids, Volatile / metabolism
-
Female
-
Ferric Compounds / administration & dosage
-
Ferric Compounds / pharmacokinetics
-
Hemoglobins / metabolism
-
Hepcidins / blood
-
Homeostasis
-
Interleukin-6 / genetics
-
Interleukin-6 / metabolism
-
Intestinal Absorption / drug effects*
-
Iron, Dietary / administration & dosage
-
Iron, Dietary / pharmacokinetics*
-
Liver / drug effects
-
Liver / metabolism
-
Micronutrients / administration & dosage
-
Micronutrients / pharmacokinetics
-
NF-kappa B / metabolism
-
PPAR gamma / genetics
-
PPAR gamma / metabolism
-
Prebiotics / analysis*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Rats
-
Rats, Wistar
-
Receptors, Transferrin / genetics
-
Receptors, Transferrin / metabolism
-
Tandem Mass Spectrometry
-
Trisaccharides / administration & dosage
-
Trisaccharides / chemistry*
Substances
-
Chlorides
-
Fatty Acids, Volatile
-
Ferric Compounds
-
Hemoglobins
-
Hepcidins
-
Interleukin-6
-
Iron, Dietary
-
Micronutrients
-
NF-kappa B
-
PPAR gamma
-
Prebiotics
-
RNA, Messenger
-
Receptors, Transferrin
-
Tfr2 protein, rat
-
Trisaccharides
-
4'-galactooligosaccharide
-
ferric chloride