Tannic acid modified silver nanoparticles show antiviral activity in herpes simplex virus type 2 infection

Piotr Orlowski; Emilia Tomaszewska; Marianna Gniadek; Piotr Baska; Julita Nowakowska; Justyna Sokolowska; Zuzanna Nowak; Mikolaj Donten; Grzegorz Celichowski; Jaroslaw Grobelny; Malgorzata Krzyzowska

doi:10.1371/journal.pone.0104113

Tannic acid modified silver nanoparticles show antiviral activity in herpes simplex virus type 2 infection

PLoS One. 2014 Aug 12;9(8):e104113. doi: 10.1371/journal.pone.0104113. eCollection 2014.

Affiliations

¹ Department of Regenerative Medicine, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
² Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lodz, Poland.
³ Faculty of Chemistry, University of Warsaw, Warsaw, Poland.
⁴ Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
⁵ Laboratory of Electron and Confocal Microscopy, Faculty of Biology, University of Warsaw, Warsaw, Poland.
⁶ Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
⁷ Department of Genetics and Animal Breeding, Faculty of Animal Science, Warsaw University of Life Sciences, Warsaw, Poland.

Abstract

The interaction between silver nanoparticles and herpesviruses is attracting great interest due to their antiviral activity and possibility to use as microbicides for oral and anogenital herpes. In this work, we demonstrate that tannic acid modified silver nanoparticles sized 13 nm, 33 nm and 46 nm are capable of reducing HSV-2 infectivity both in vitro and in vivo. The antiviral activity of tannic acid modified silver nanoparticles was size-related, required direct interaction and blocked virus attachment, penetration and further spread. All tested tannic acid modified silver nanoparticles reduced both infection and inflammatory reaction in the mouse model of HSV-2 infection when used at infection or for a post-infection treatment. Smaller-sized nanoparticles induced production of cytokines and chemokines important for anti-viral response. The corresponding control buffers with tannic acid showed inferior antiviral effects in vitro and were ineffective in blocking in vivo infection. Our results show that tannic acid modified silver nanoparticles are good candidates for microbicides used in treatment of herpesvirus infections.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiviral Agents / chemistry
Antiviral Agents / pharmacology*
Cell Line
Chlorocebus aethiops
Cytokines / metabolism
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Herpes Simplex / drug therapy*
Herpes Simplex / metabolism
Herpes Simplex / virology*
Herpesvirus 2, Human / drug effects*
Inflammation / drug therapy
Inflammation / metabolism
Inflammation / virology
Metal Nanoparticles / administration & dosage*
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Mice
Microbial Sensitivity Tests
Silver / chemistry*
Silver / pharmacology*
Tannins / chemistry*
Virus Attachment / drug effects
Virus Internalization / drug effects

Substances

Antiviral Agents
Cytokines
Tannins
Silver

Grants and funding

This work was supported by the Polish National Science Centre grant No. 2011/03/B/NZ6/04878 (for MK) and Centre for Preclinical Research and Technology (CePT) Project No. POIG.02.02.00-14-024/08-0 (for MG and MD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.