6,6'-Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3-L1 cells

J Sci Food Agric. 2015 Jul;95(9):1830-7. doi: 10.1002/jsfa.6881. Epub 2014 Oct 28.

Abstract

Background: Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown.

Results: We investigated the anti-obesity effects of 6,6'-bieckol, 6,8'-bieckol, 8,8'-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCATT/enhancer-binding protein α (C/EBPα) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50 µg mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6'-bieckol markedly decreased lipid accumulation and expression levels of PPARγ, C/EBPα, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA).

Conclusion: These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis.

Keywords: 3T3-L1; adipogenesis; anti-obesity; lipogenesis; phlorotannins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipogenesis / drug effects*
  • Animals
  • Anti-Obesity Agents / adverse effects
  • Anti-Obesity Agents / chemistry
  • Anti-Obesity Agents / isolation & purification
  • Anti-Obesity Agents / pharmacology*
  • Benzofurans / adverse effects
  • Benzofurans / chemistry
  • Benzofurans / isolation & purification
  • Benzofurans / pharmacology
  • Carbon-Carbon Ligases / antagonists & inhibitors
  • Carbon-Carbon Ligases / genetics
  • Carbon-Carbon Ligases / metabolism
  • Cell Survival / drug effects
  • Dioxins / adverse effects
  • Dioxins / chemistry
  • Dioxins / isolation & purification
  • Dioxins / pharmacology*
  • Down-Regulation / drug effects*
  • Fatty Acid Synthase, Type I / antagonists & inhibitors
  • Fatty Acid Synthase, Type I / genetics
  • Fatty Acid Synthase, Type I / metabolism
  • Lipid Metabolism / drug effects*
  • Mice
  • Molecular Structure
  • PPAR gamma / antagonists & inhibitors
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Pacific Ocean
  • Phaeophyceae / chemistry
  • Republic of Korea
  • Seaweed / chemistry
  • Stereoisomerism

Substances

  • Anti-Obesity Agents
  • Benzofurans
  • Dioxins
  • PPAR gamma
  • dieckol
  • phlorofucofuroeckol A
  • 6,6'-bieckol
  • Fatty Acid Synthase, Type I
  • Carbon-Carbon Ligases
  • acyl-CoA carboxylase