Abstract
We investigated the reversal effect of vitamin D on the multidrug-resistant leukemic Jurkat/ADR and K562/ADR cell lines and conducted a preliminary investigation of its reversal mechanism. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the reversal effect of vitamin D on multidrug-resistant cells. Real-time polymerase chain reaction was used to determine the effect of vitamin D on intracellular expression of mRNA of the multidrug-resistant gene (MDRI) and the multidrug-resistance-related gene (MRP1). A protein quantitative analysis method was used to determine the effect of vitamin D on intracellular glutathione content. After treatment of Jurkat/ADR and K562/ADR cells with vitamin D, multidrug resistance was reversed in a dose-dependent manner, which may have reduced mRNA expression of the MDR1 and MRP1 genes, the P-glycoprotein content on the cell surface, and the intracellular glutathione level. Different concentrations of vitamin D showed varying reversal effects on different multidrug-resistant cells. The resistance mechanism may be related to the inhibition of the expression of MDR1 and MRP1 genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B / antagonists & inhibitors
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ATP Binding Cassette Transporter, Subfamily B / genetics
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ATP Binding Cassette Transporter, Subfamily B / metabolism
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Antineoplastic Agents / pharmacology*
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Cell Survival / drug effects
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Cytarabine / pharmacology
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Doxorubicin / pharmacology
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Drug Resistance, Neoplasm / drug effects*
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Drug Resistance, Neoplasm / genetics
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Gene Expression Regulation, Leukemic*
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Glutathione / metabolism
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Humans
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Inhibitory Concentration 50
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Jurkat Cells
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K562 Cells
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Multidrug Resistance-Associated Proteins / antagonists & inhibitors*
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Multidrug Resistance-Associated Proteins / genetics
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Multidrug Resistance-Associated Proteins / metabolism
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RNA, Messenger / antagonists & inhibitors*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Signal Transduction
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Vincristine / pharmacology
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Vitamin D / pharmacology*
Substances
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ABCB1 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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Antineoplastic Agents
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Multidrug Resistance-Associated Proteins
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RNA, Messenger
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Cytarabine
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Vitamin D
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Vincristine
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Doxorubicin
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Glutathione
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multidrug resistance-associated protein 1