Profiling metabolic states with genetically encoded fluorescent biosensors for NADH

Yuzheng Zhao; Yi Yang

doi:10.1016/j.copbio.2014.08.007

Profiling metabolic states with genetically encoded fluorescent biosensors for NADH

Curr Opin Biotechnol. 2015 Feb:31:86-92. doi: 10.1016/j.copbio.2014.08.007. Epub 2014 Sep 29.

Authors

Yuzheng Zhao¹, Yi Yang²

Affiliations

¹ Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
² Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address: yiyang@ecust.edu.cn.

PMID: 25269782
DOI: 10.1016/j.copbio.2014.08.007

Abstract

NADH and its oxidized form, NAD(+), play central roles in energy metabolism and are ideal indicators of cellular metabolic states. In this review, we will introduce recent progress made in the developing of a series of genetically encoded NADH sensors, which offer the potential to fill the gap in currently used techniques of endogenous NAD(P)H fluorescence imaging. These sensors are bright, specific and organelles targetable, allowing real-time tracking and quantification of intracellular NADH levels in different subcellular compartments. The individual strengths and weaknesses of these sensors when applied to the study of metabolic states profiling will be also discussed.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Biosensing Techniques / methods*
Fluorescence
Humans
Metabolomics / methods*
NAD / genetics
NAD / metabolism
Oxidation-Reduction

Substances

NAD