A simple method for the separation of Bacillus thuringiensis spores and crystals

Jihane Rahbani Mounsef; Dominique Salameh; Mireille kallassy Awad; Laure Chamy; Cedric Brandam; Roger Lteif

doi:10.1016/j.mimet.2014.10.003

A simple method for the separation of Bacillus thuringiensis spores and crystals

J Microbiol Methods. 2014 Dec:107:147-9. doi: 10.1016/j.mimet.2014.10.003.

Authors

Jihane Rahbani Mounsef¹, Dominique Salameh, Mireille kallassy Awad, Laure Chamy, Cedric Brandam, Roger Lteif

Affiliation

¹ Université de Toulouse, INPT, UPS, Laboratoire de Génie Chimique, 4, Allée Emile Monso, F-31030 Toulouse, France. gihane.rahbany@usj.edu.lb

PMID: 25315286
DOI: 10.1016/j.mimet.2014.10.003

Abstract

A simple new method, for separating Bacillus thuringiensis crystals from spores and cell debris, is described. The developed purification method uses hexane and low speed centrifugation and does not require any expensive material or reagents.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus thuringiensis*
Bacterial Proteins / chemistry
Bacterial Proteins / isolation & purification*
Spores, Bacterial / isolation & purification*

Substances

Bacterial Proteins