A reduction and pH dual-sensitive polymeric vector for long-circulating and tumor-targeted siRNA delivery

Adv Mater. 2014 Dec 23;26(48):8217-24. doi: 10.1002/adma.201403877. Epub 2014 Oct 31.

Abstract

A novel reduction and pH dual-sensitive nonviral vector for long-circulating and tumor-targeted siRNA delivery is described. The nanomedicine is negatively charged at neutral pH of bloodstream whereas it is positively charged at lower pH of tumor tissue (ca. 6.8). Interlayer crosslinking with disulfide bonds stabilizes the nanomedicine during blood circulation and allows quick intracellular siRNA release after endocytosis.

Keywords: cancer treatment; charge reversal; micelles; reducible; siRNA delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbocyanines / chemistry
  • Cell Line, Tumor
  • Endocytosis
  • Genetic Vectors / chemistry
  • Genetic Vectors / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Mice
  • Mice, Nude
  • Microscopy, Confocal
  • Neoplasms / diagnosis
  • Neoplasms / pathology
  • Optical Imaging
  • Oxidation-Reduction
  • Polyethylene Glycols / chemistry
  • Polymers / chemistry*
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / metabolism*
  • Spectroscopy, Fourier Transform Infrared
  • Transfection
  • Transplantation, Heterologous

Substances

  • Carbocyanines
  • Polymers
  • RNA, Small Interfering
  • cyanine dye 3
  • Polyethylene Glycols