Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects

Shichang Zhao; Jianhua Zhang; Min Zhu; Yadong Zhang; Zhongtang Liu; Cuilian Tao; Yufang Zhu; Changqing Zhang

doi:10.1016/j.actbio.2014.10.015

Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects

Acta Biomater. 2015 Jan:12:270-280. doi: 10.1016/j.actbio.2014.10.015. Epub 2014 Oct 23.

Authors

Shichang Zhao¹, Jianhua Zhang², Min Zhu³, Yadong Zhang¹, Zhongtang Liu⁴, Cuilian Tao², Yufang Zhu⁵, Changqing Zhang⁶

Affiliations

¹ Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China.
² School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China; School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
³ School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China.
⁴ Department of Orthopedics, Changhai Hospital, Second Military Medical University, 174 Changhai Road, Shanghai 200433, People's Republic of China.
⁵ School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, People's Republic of China. Electronic address: zjf2412@163.com.
⁶ Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China. Electronic address: shzhangchangqing@163.com.

PMID: 25449915
DOI: 10.1016/j.actbio.2014.10.015

Abstract

The development of a new generation of biomaterials with high osteogenic ability for fast osseointegration with host bone is being intensively investigated. In this study, we have fabricated three-dimensional (3-D) strontium-containing mesoporous bioactive glass (Sr-MBG) scaffolds by a 3-D printing technique. Sr-MBG scaffolds showed uniform interconnected macropores (∼400μm), high porosity (∼70%) and enhanced compressive strength (8.67±1.74MPa). Using MBG scaffolds as a control, the biological properties of Sr-MBG scaffolds were evaluated by apatite-forming ability, adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteoblast-like cells MC3T3-E1. Furthermore, Sr-MBG scaffolds were used to repair critical-sized rat calvarial defects. The results showed that Sr-MBG scaffolds possessed good apatite-forming ability and stimulated MC3T3-E1 cell proliferation and differentiation. Importantly, the in vivo results revealed that Sr-MBG scaffolds had good osteogenic capability and stimulated new blood vessel formation in critical-sized rat calvarial defects within 8 weeks. Therefore, 3-D printed Sr-MBG scaffolds with favorable pore structure and high osteogenic ability have more potential applications in bone regeneration.

Keywords: 3-D printing; Bone regeneration; In vivo; Mesoporous bioactive glass; Strontium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Animals
Biocompatible Materials*
Glass*
Mice
Microscopy, Electron, Scanning
Osteoblasts / cytology
Rats
Skull / abnormalities*
Strontium / chemistry*
Tissue Scaffolds*
X-Ray Microtomography

Substances

Biocompatible Materials
Strontium