Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b

Gleb Aktuganov; Jouni Jokela; Henri Kivelä; Elvira Khalikova; Alexander Melentjev; Nailia Galimzianova; Lyudmila Kuzmina; Petri Kouvonen; Juha-Pekka Himanen; Petri Susi; Timo Korpela

doi:10.1016/j.jchromb.2014.09.042

Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b

J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Dec 15:973C:9-16. doi: 10.1016/j.jchromb.2014.09.042. Epub 2014 Oct 8.

Authors

Affiliations

¹ Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia. Electronic address: gleakt@anrb.ru.
² Division of Microbiology, Department of Food and Environmental Sciences, University of Helsinki, Viikki Biocenter, P.O. Box 56, 00014 Helsinki, Finland.
³ Department of Chemistry, University of Turku, 20014 Turku, Finland.
⁴ Technology Research Centre, University of Turku, 20520 Turku, Finland.
⁵ Institute of Biology, Ufa Research Centre of Russian Academy of Sciences, 450054 Ufa, Russia.
⁶ Turku Centre for Biotechnology, University of Turku and Ǻbo Academi, 20520 Turku, Finland. Electronic address: petri.kouvonen@btk.fi.
⁷ Structural Biology Program, Sloan-Kettering Institute for Cancer Research, 10021 NY, United States.
⁸ Department of Virology, Institute of Microbiology and Pathology, University of Turku, 20520 Turku, Finland.

PMID: 25464089
DOI: 10.1016/j.jchromb.2014.09.042

Abstract

Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water-methanol extraction followed by a chromatography on a C₁₈-support. The analysis with LC-MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C₁₅, fengycin/plipastatin A-C₁₆ together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.

Keywords: Antifungal compounds; Biocontrol; Cyclic lipopeptides; LC–MS; Microbial antagonism; Paenibacillus ehimensis; Purification.