Abstract
Curcumin is a yellow pigment isolated from Corcuma longan. This research investigates the improvement of curcumin on INS-1 cells with insulin resistance induced by high glucose. INS-1 cells were treated with high glucose (30 mmol L(-1)) for 48 h. Subsequently, the medium was replaced with curcumin for 24 h. Curcumin effectively increased insulin gene expression and glucose stimulated insulin secretion (GSIS) in a dose-dependent manner. Furthermore, the molecular mechanism of curcumin-induced insulin expression and secretion in high glucose-induced INS-1 cells was investigated in this study. Curcumin increased the expression of glucose transporter 2 (GLUT2) and phosphorylation of insulin receptor (IR), insulin receptor substrate-1 (IRS1), phosphatidylinositol-3-kinase (PI3K) and AKT in the INS-1 cells. Moreover, curcumin stimulation increased the expression of PDX-1 and GCK. This investigation suggests that curcumin prevented high glucose-reduced insulin expression and secretion through activation of the PI3K/Akt/GLUT2 pathway in INS-1 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cell Survival / drug effects
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Curcumin / pharmacology*
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Glucokinase / genetics
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Glucokinase / metabolism
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Glucose / administration & dosage
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Glucose / adverse effects*
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Glucose / analysis
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Glucose Transporter Type 2 / genetics
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Glucose Transporter Type 2 / metabolism
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Insulin / metabolism*
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Insulin Receptor Substrate Proteins / genetics
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Insulin Receptor Substrate Proteins / metabolism
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Insulin Resistance
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Insulin Secretion
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Insulin-Secreting Cells / drug effects*
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Phosphatidylinositol 3-Kinases / genetics
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Phosphatidylinositol 3-Kinases / metabolism
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Phosphorylation
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Proto-Oncogene Proteins c-akt / genetics
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Receptor, Insulin / genetics
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Receptor, Insulin / metabolism
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Up-Regulation
Substances
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Glucose Transporter Type 2
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Homeodomain Proteins
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Insulin
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Insulin Receptor Substrate Proteins
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RNA, Messenger
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Slc2a2 protein, rat
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Trans-Activators
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pancreatic and duodenal homeobox 1 protein
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Phosphatidylinositol 3-Kinases
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Glucokinase
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Receptor, Insulin
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Proto-Oncogene Proteins c-akt
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Curcumin
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Glucose