The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

Reprod Biomed Online. 2015 Feb;30(2):203-7. doi: 10.1016/j.rbmo.2014.10.008. Epub 2014 Oct 22.

Abstract

In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool.

Keywords: IVF; embryo quality; follicular fluid; in-vitro maturation; oocyte developmental competence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / metabolism*
  • Cattle
  • Embryo Culture Techniques / methods*
  • Female
  • Fertilization in Vitro / methods*
  • Follicular Fluid / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Humans
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / metabolism
  • Lactate Dehydrogenase 5
  • Oocytes / cytology
  • Oocytes / drug effects*
  • Transcription Factors / metabolism

Substances

  • Isoenzymes
  • Transcription Factors
  • peroxisome-proliferator-activated receptor-gamma coactivator-1
  • L-Lactate Dehydrogenase
  • Lactate Dehydrogenase 5