A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies

Qi Wang; Xiaofei Li; Xiaolin Ji; Jingfei Wang; Nan Shen; Yulong Gao; Xiaole Qi; Yongqiang Wang; Honglei Gao; Shide Zhang; Xiaomei Wang

doi:10.1371/journal.pone.0115422

A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies

PLoS One. 2014 Dec 18;9(12):e115422. doi: 10.1371/journal.pone.0115422. eCollection 2014.

Authors

Affiliations

¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
² Centre for Animal Infectious Disease Diagnosis and Technical Services and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
³ Departments of Radiology, Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China.

Abstract

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / genetics*
Antibodies, Neutralizing / immunology
Antibodies, Viral / genetics
Antibodies, Viral / immunology
Avian Leukosis / immunology
Avian Leukosis / virology
Avian Leukosis Virus / genetics
Avian Leukosis Virus / immunology*
Cell Line, Tumor
Chickens
Neutralization Tests / methods*

Substances

Antibodies, Neutralizing
Antibodies, Viral

Grants and funding

This study was funded by the National Natural Science Foundation of China (31072146), the National Natural Science Foundation of China (31201923) and the earmarked fund for the Modern Agro-industry Technology Research System (no. nycytx-42-G3-01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.