Monitoring the cytosolic entry of cell-penetrating peptides using a pH-sensitive fluorophore

Chem Commun (Camb). 2015 Feb 7;51(11):2162-5. doi: 10.1039/c4cc09441g.

Abstract

We report a simple, effective method to assess the cytosolic delivery efficiency and kinetics of cell-penetrating peptides using a pH-sensitive fluorescent probe, naphthofluorescein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell-Penetrating Peptides / metabolism*
  • Cytosol / metabolism*
  • Flow Cytometry
  • Fluorescein / chemistry*
  • Fluoresceins / chemistry*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Protein Transport
  • Spectrometry, Fluorescence

Substances

  • Cell-Penetrating Peptides
  • Fluoresceins
  • Fluorescent Dyes
  • naphthofluorescein
  • Fluorescein