Abstract
Gene encoding 22-380 aa of spike protein of porcine epidemic diarrhea virus (PEDV) isolate in China was cloned and expressed as a recombinant protein in Escherichia coli BL21(DE3). Female BALB/c mice were immunized with the purified recombinant S protein, and a monoclonal antibody (MAb) designated as 2D1 against S protein was achieved by hybridoma technique. MAb 2D1 reacted with S protein of PEDV specifically. The monoclonal antibody 2D1 may provide a useful tool as a specific diagnostic reagent for detecting S protein of the Chinese variant of PEDV and for further investigation into the virus' pathogenic mechanism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / isolation & purification
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Antibodies, Viral / biosynthesis*
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Antibodies, Viral / isolation & purification
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Cell Line
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Coronavirus Infections / diagnosis*
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Coronavirus Infections / immunology
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Coronavirus Infections / veterinary*
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Coronavirus Infections / virology
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Female
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Founder Effect
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Gene Expression
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Hybridomas / immunology
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Mice
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Mice, Inbred BALB C
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Porcine epidemic diarrhea virus / immunology
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Porcine epidemic diarrhea virus / isolation & purification*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Spike Glycoprotein, Coronavirus / chemistry
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Spike Glycoprotein, Coronavirus / genetics
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Spike Glycoprotein, Coronavirus / immunology*
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Swine
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Swine Diseases / diagnosis*
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Swine Diseases / immunology
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Swine Diseases / virology
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Recombinant Proteins
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Spike Glycoprotein, Coronavirus