Abstract
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract. This chapter is focusing on the preparation of cryogels as monolithic column and immobilization of concanavalin A on its surface as ligand for capturing the glycoprotein horseradish peroxidase.
MeSH terms
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Chromatography, Affinity / methods*
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Concanavalin A / chemistry
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Cryogels / chemistry*
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Horseradish Peroxidase / chemistry
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Horseradish Peroxidase / isolation & purification
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Immobilized Proteins / chemistry
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Ligands
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Nanoparticles / chemistry
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Polyvinyl Alcohol / chemistry
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Proteins / chemistry
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Proteins / isolation & purification*
Substances
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Cryogels
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Immobilized Proteins
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Ligands
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Proteins
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Concanavalin A
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Polyvinyl Alcohol
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Horseradish Peroxidase