Simultaneous capture, detection, and inactivation of bacteria as enabled by a surface-enhanced Raman scattering multifunctional chip

Angew Chem Int Ed Engl. 2015 Apr 20;54(17):5132-6. doi: 10.1002/anie.201412294. Epub 2015 Mar 27.

Abstract

Herein, we present a multifunctional chip based on surface-enhanced Raman scattering (SERS) that effectively captures, discriminates, and inactivates pathogenic bacteria. The developed SERS chip is made of a silicon wafer decorated with silver nanoparticles and modified with 4-mercaptophenylboronic acid (4-MPBA). It was prepared in a straightforward manner by chemical reduction assisted by hydrogen fluoride etching, followed by the conjugation of 4-MPBA through AgS bonds. The dominant merits of the fabricated SERS chip include excellent reproducibility with a relative standard deviation (RSD) value smaller than 11.0 %, adaptable bacterial-capture efficiency (ca. 60 %) at low concentrations (500-2000 CFU mL(-1) ), a low detection limit (down to a concentration of 1.0×10(2) cells mL(-1) ), and high antibacterial activity (an antibacterial rate of ca. 97 %). The SERS chip enabled sensitive and specific discrimination of Escherichia coli and Staphylococcus aureus from human blood.

Keywords: bacteria; multifunctional platforms; silicon; silver nanoparticles; surface-enhanced Raman scattering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / instrumentation*
  • Boronic Acids / chemistry
  • Escherichia coli / chemistry
  • Escherichia coli / isolation & purification*
  • Humans
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Microscopy, Atomic Force
  • Plasma / microbiology
  • Silver / chemistry*
  • Spectrum Analysis, Raman*
  • Staphylococcus aureus / chemistry
  • Staphylococcus aureus / isolation & purification*
  • Sulfhydryl Compounds / chemistry

Substances

  • 4-mercaptophenylboronic acid
  • Boronic Acids
  • Sulfhydryl Compounds
  • Silver