A rationally designed peptidomimetic biosensor for sialic acid on cell surfaces

Chem Commun (Camb). 2015 May 11;51(38):8112-5. doi: 10.1039/c5cc01662b. Epub 2015 Apr 14.

Abstract

We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia composition on the cell surfaces and filopodia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / chemistry
  • Biosensing Techniques*
  • Biotin / chemistry
  • Boronic Acids / chemistry
  • Cell Line, Tumor
  • Fluorescence
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Structure
  • N-Acetylneuraminic Acid / analysis*
  • NIH 3T3 Cells
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry*
  • Peptidomimetics*
  • Streptavidin / chemistry
  • Surface Properties

Substances

  • Boronic Acids
  • Oligopeptides
  • Peptidomimetics
  • Biotin
  • Streptavidin
  • Arginine
  • N-Acetylneuraminic Acid
  • benzeneboronic acid