Abstract
We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to fluorescent streptavidin via biotin enabling the optical labeling of cells. This approach provides unique opportunities to detect Sia composition on the cell surfaces and filopodia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arginine / chemistry
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Biosensing Techniques*
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Biotin / chemistry
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Boronic Acids / chemistry
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Cell Line, Tumor
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Fluorescence
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HeLa Cells
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Humans
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Mice
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Molecular Structure
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N-Acetylneuraminic Acid / analysis*
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NIH 3T3 Cells
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Oligopeptides / chemical synthesis
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Oligopeptides / chemistry*
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Peptidomimetics*
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Streptavidin / chemistry
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Surface Properties
Substances
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Boronic Acids
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Oligopeptides
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Peptidomimetics
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Biotin
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Streptavidin
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Arginine
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N-Acetylneuraminic Acid
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benzeneboronic acid