A bioelectronic nose based on a novel microfluidic system (μBN) was fabricated to detect gaseous trimethylamine (TMA) in real-time. Single-walled carbon nanotube-field effect transistors (SWNT-FETs) were functionalized with olfactory receptor-derived peptides (ORPs) that can recognize the TMA molecules. The ORP-coated SWNT-FETs were assembled with a microfluidic channel and were sealed with top and bottom frames. This simple process was used to complete the μBNs, and a well-defined condition was achieved to detect the gaseous molecules. The μBNs allowed us to detect gaseous TMA molecules down to 10 parts per trillion (ppt) in real-time and showed high selectivity when distinguishing gaseous TMA from other gaseous odorants. The sensor was used to determine the quality of seafood (oysters), and spoiled seafood and other types of spoiled foods were also successfully discriminated without any pretreatment processes. These results indicate that portable-scale platforms can be manufactured by using μBNs and can be applicable for real-time on-site gas analysis.
Keywords: Bioelectronic nose; Carbon nanotube; Microfluidic gas sensor; Olfactory receptor; Trimethylamine.
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