Image distortion and its correction in linear galvanometric mirrors-based laser-scanning microscopy

J Biomed Opt. 2015 May;20(5):56001. doi: 10.1117/1.JBO.20.5.056001.

Abstract

To simplify imaging focusing and calibration tasks, a laser-scanning microscope needs to scan ata moderate frame rate. The inertia of a galvanometric scanner leads to time delays when following external commands, which subsequently introduces image distortions that deteriorate as scan frequency increases. Sinusoidal and triangular waveforms were examined as fast axis driving patterns. The interplay among driving pattern, frequency, sampling rate, phase shift, linear scanning range, and their effect on reconstructed images was discussed. Utilizing position feedback from the linear galvo scanners, the effect of response time could be automatically compensated in real time. Precompensated triangular driving waveform offered the least amount of image distortion.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Artifacts*
  • Equipment Design
  • Equipment Failure Analysis
  • Feedback
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods*
  • Lenses*
  • Micro-Electrical-Mechanical Systems / instrumentation*
  • Microscopy, Confocal / instrumentation*
  • Phantoms, Imaging
  • Reproducibility of Results
  • Sensitivity and Specificity