Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene

Proc Natl Acad Sci U S A. 1989 Dec;86(23):9119-23. doi: 10.1073/pnas.86.23.9119.

Abstract

We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / genetics*
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Chromosome Deletion
  • Gene Expression
  • Genes
  • Genes, Bacterial*
  • Leishmania mexicana / genetics*
  • Molecular Sequence Data
  • Plasmids
  • Recombinant Proteins / metabolism
  • Transcription, Genetic
  • Transfection*
  • Tubulin / genetics

Substances

  • Recombinant Proteins
  • Tubulin
  • Chloramphenicol O-Acetyltransferase