Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

Tianlin Gao; Ning Zhang; Zongliang Wang; Yu Wang; Ya Liu; Yoshihiro Ito; Peibiao Zhang

doi:10.1002/mabi.201500069

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

Macromol Biosci. 2015 Aug;15(8):1070-80. doi: 10.1002/mabi.201500069. Epub 2015 May 7.

Authors

Tianlin Gao^{1

2}, Ning Zhang³, Zongliang Wang², Yu Wang², Ya Liu⁴, Yoshihiro Ito^{5

6}, Peibiao Zhang⁷

Affiliations

¹ School of Public Health, Jilin University, Changchun, 130021, P. R. China.
² Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
³ Department of Foot and Ankle Surgery, The Second Hospital of Shandong University, Jinan, 250000, P. R. China.
⁴ School of Public Health, Jilin University, Changchun, 130021, P. R. China. liuya@jlu.edu.cn.
⁵ Nano Medical Engineering Laboratory, RIKEN, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.
⁶ Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Materials Science, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.
⁷ Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. zhangpb@ciac.ac.cn.

PMID: 25950171
DOI: 10.1002/mabi.201500069

Abstract

In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20 min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.

Keywords: IGF-1; biodegradable PLGA microcarriers; bone tissue engineering; nano-hydroxyapatite; polydopamine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / cytology
Alkaline Phosphatase / biosynthesis
Animals
Biodegradable Plastics / chemistry*
Biodegradable Plastics / pharmacology
Cell Differentiation / drug effects*
Cell Proliferation / drug effects*
Durapatite / chemistry
Durapatite / pharmacology
Immobilized Proteins / chemistry
Immobilized Proteins / pharmacology
Indoles / chemistry
Insulin-Like Growth Factor I / chemistry*
Insulin-Like Growth Factor I / metabolism
Mice
Osteogenesis / drug effects*
Polyglactin 910 / chemistry
Polyglactin 910 / pharmacology
Polymers / chemistry
Stem Cells / drug effects

Substances

Biodegradable Plastics
Immobilized Proteins
Indoles
Polymers
polydopamine
Polyglactin 910
Insulin-Like Growth Factor I
Durapatite
Alkaline Phosphatase