Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

Yuan Zhou; Hui Wang; Cong Wang; Xuefeng Qiu; Mikael Benson; Xiaoqin Yin; Zou Xiang; Dongmei Li; Xiaodong Han

doi:10.1016/j.taap.2015.05.008

Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

Toxicol Appl Pharmacol. 2015 Aug 15;287(1):1-8. doi: 10.1016/j.taap.2015.05.008. Epub 2015 May 15.

Authors

Yuan Zhou¹, Hui Wang², Cong Wang¹, Xuefeng Qiu³, Mikael Benson², Xiaoqin Yin¹, Zou Xiang⁴, Dongmei Li⁵, Xiaodong Han⁶

Affiliations

¹ Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China.
² The Centre for Individualized Medication, Linköping University Hospital, Linköping University, Linköping SE-58185, Sweden.
³ Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, PR China.
⁴ Department of Microbiology and Immunology, Mucosal Immunobiology and Vaccine Research Center, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
⁵ Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China. Electronic address: lidm@nju.edu.cn.
⁶ Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu 210093, PR China. Electronic address: hanxd@nju.edu.cn.

PMID: 25986756
DOI: 10.1016/j.taap.2015.05.008

Abstract

Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system.

Keywords: Bioinformatics; Microcystin-LR; Sertoli cell; Toxicity; mRNA; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Computational Biology
Dose-Response Relationship, Drug
Gene Expression Profiling
Gene Expression Regulation
Male
Marine Toxins
Mice, Inbred BALB C
MicroRNAs / metabolism*
Microcystins / toxicity*
RNA, Messenger / metabolism
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sertoli Cells / drug effects*
Sertoli Cells / metabolism
Sertoli Cells / pathology

Substances

Marine Toxins
MicroRNAs
Microcystins
RNA, Messenger
cyanoginosin LR