Canine parvoviruses in New Zealand form a monophyletic group distinct from the viruses circulating in other parts of the world

Vet Microbiol. 2015 Aug 5;178(3-4):190-200. doi: 10.1016/j.vetmic.2015.05.017. Epub 2015 May 27.

Abstract

Canine parvovirus 2 (CPV-2) is a well-recognized cause of acute haemorrhagic enteritis in dogs worldwide. The aim of the current study was to identify which CPV-2 subtypes circulate among dogs in New Zealand, and to investigate the evolutionary patterns of contemporary CPV-2 viruses. Faecal samples were collected from 79 dogs with suspected CPV-2 infection over the period of 13 months, and tested for the presence of CPV-2 DNA by PCR. Of 70 positive samples, 69 were subtyped as CPV-2a and one as CPV-2. A majority of CPV-2 positive samples were collected from unvaccinated or not-fully vaccinated puppies ≤6 months of age. The haplotype network produced from New Zealand CPV-2 sequences showed no structure when assessed based on location, vaccination status or age of the animals sampled. International haplotype network indicated that, unlike CPV-2 from other countries, the population of CPV-2 in New Zealand appeared to be monophyletic.

Keywords: CPV-2; CPV-2 evolution; Canine parvovirus; Canine vaccination; Haplotype network; Molecular typing; PCR; Parvovirus enteritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Dog Diseases / epidemiology
  • Dog Diseases / virology*
  • Dogs
  • Enteritis / epidemiology
  • Enteritis / veterinary*
  • Enteritis / virology
  • Feces / virology
  • Female
  • Haplotypes
  • New Zealand / epidemiology
  • Parvoviridae Infections / epidemiology
  • Parvoviridae Infections / veterinary*
  • Parvoviridae Infections / virology
  • Parvovirus, Canine / genetics*
  • Parvovirus, Canine / isolation & purification