The molecular basis for flexibility in the flexible filamentous plant viruses

Frank DiMaio; Chun-Chieh Chen; Xiong Yu; Brandon Frenz; Yau-Heiu Hsu; Na-Sheng Lin; Edward H Egelman

doi:10.1038/nsmb.3054

The molecular basis for flexibility in the flexible filamentous plant viruses

Nat Struct Mol Biol. 2015 Aug;22(8):642-4. doi: 10.1038/nsmb.3054. Epub 2015 Jul 13.

Authors

Frank DiMaio¹, Chun-Chieh Chen², Xiong Yu³, Brandon Frenz¹, Yau-Heiu Hsu⁴, Na-Sheng Lin⁵, Edward H Egelman³

Affiliations

¹ Department of Biochemistry, University of Washington, Seattle, Washington, USA.
² 1] Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. [2] Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan. [3] Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
³ Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA.
⁴ Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.
⁵ 1] Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. [2] Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan. [3] Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan.

Abstract

Flexible filamentous plant viruses cause more than half the viral crop damage in the world but are also potentially useful for biotechnology. Structural studies began more than 75 years ago but have failed, owing to the virion's extreme flexibility. We have used cryo-EM to generate an atomic model for bamboo mosaic virus, which reveals flexible N- and C-terminal extensions that allow deformation while still maintaining structural integrity.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Bambusa / virology*
Capsid / chemistry
Capsid / metabolism
Capsid Proteins / chemistry*
Capsid Proteins / genetics
Capsid Proteins / metabolism
Cryoelectron Microscopy
Crystallography, X-Ray
Models, Molecular
Mosaic Viruses / chemistry*
Mosaic Viruses / genetics
Mosaic Viruses / ultrastructure
Protein Binding
Protein Structure, Secondary
Protein Structure, Tertiary
RNA, Viral / chemistry*
RNA, Viral / genetics
RNA, Viral / metabolism
Virion / chemistry*
Virion / genetics
Virion / ultrastructure

The molecular basis for flexibility in the flexible filamentous plant viruses

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