Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles

Ciro Chiappini; Paola Campagnolo; Carina S Almeida; Nima Abbassi-Ghadi; Lesley W Chow; George B Hanna; Molly M Stevens

doi:10.1002/adma.201501304

Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles

Adv Mater. 2015 Sep 16;27(35):5147-52. doi: 10.1002/adma.201501304. Epub 2015 Jul 21.

Authors

Ciro Chiappini¹, Paola Campagnolo¹, Carina S Almeida¹, Nima Abbassi-Ghadi², Lesley W Chow¹, George B Hanna², Molly M Stevens¹

Affiliations

¹ Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
² Department of Surgery and Cancer, Imperial College London, W2 1PG, London, UK.

Abstract

Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma.

Keywords: diagnostics; intracellular sensing; nanomedicine; nanoneedles; porous silicon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Cathepsin B / metabolism*
Cell Line, Tumor
Cytosol / enzymology*
Esophagus / cytology*
Humans
Mucous Membrane / cytology
Nanotechnology / methods*
Porosity
Silicon / chemistry*

Substances

CTSB protein, human
Cathepsin B
Silicon

Abstract

Publication types

MeSH terms

Substances

Grants and funding