Stiffness-Independent Highly Efficient On-Chip Extraction of Cell-Laden Hydrogel Microcapsules from Oil Emulsion into Aqueous Solution by Dielectrophoresis

Haishui Huang; Mingrui Sun; Tyler Heisler-Taylor; Asimina Kiourti; John Volakis; Gregory Lafyatis; Xiaoming He

doi:10.1002/smll.201501388

Stiffness-Independent Highly Efficient On-Chip Extraction of Cell-Laden Hydrogel Microcapsules from Oil Emulsion into Aqueous Solution by Dielectrophoresis

Small. 2015 Oct 28;11(40):5369-74. doi: 10.1002/smll.201501388. Epub 2015 Aug 21.

Authors

Haishui Huang¹, Mingrui Sun², Tyler Heisler-Taylor², Asimina Kiourti³, John Volakis³, Gregory Lafyatis⁴, Xiaoming He²

Affiliations

¹ Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, 43210, USA.
² Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.
³ Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH, 43210, USA.
⁴ Department of Physics, The Ohio State University, Columbus, OH, 43210, USA.

Abstract

A dielectrophoresis (DEP)-based method achieves highly efficient on-chip extraction of cell-laden microcapsules of any stiffness from oil into aqueous solution. The hydrogel microcapsules can be extracted into the aqueous solution by DEP and interfacial tension forces with no trapped oil, while the encapsulated cells are free from electrical damage due to the Faraday cage effect.

Keywords: dielectrophoresis; hydrogels, microcapsules; interfacial tension; liquid electrodes; on-chip extraction.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Capsules / chemistry*
Emulsions / chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*

Substances

Capsules
Emulsions
Hydrogel, Polyethylene Glycol Dimethacrylate

Abstract

Publication types

MeSH terms

Substances

Grants and funding