Identification and genetic characterization of two porcine astroviruses from domestic piglets in China

Arch Virol. 2015 Dec;160(12):3079-84. doi: 10.1007/s00705-015-2569-x. Epub 2015 Sep 4.

Abstract

Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82% (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.

Keywords: Domestic pig; Faeces; Porcine astrovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Domestic / virology
  • Astroviridae Infections / veterinary*
  • Astroviridae Infections / virology
  • China
  • Feces / virology
  • Genotype
  • Mamastrovirus / classification
  • Mamastrovirus / genetics*
  • Mamastrovirus / isolation & purification*
  • Molecular Sequence Data
  • Phylogeny
  • Swine
  • Swine Diseases / virology*

Associated data

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