One-Step Identification of Five Prominent Chicken Salmonella Serovars and Biotypes

J Clin Microbiol. 2015 Dec;53(12):3881-3. doi: 10.1128/JCM.01976-15. Epub 2015 Sep 16.

Abstract

Based on bacterial genomic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultaneously differentiate the two invasive avian-adapted S. enterica serovar Gallinarum biotypes Gallinarum and Pullorum, and the most frequent, specific, and asymptomatic colonizers of chickens, serovars Enteritidis, Heidelberg, and Kentucky.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacteriological Techniques / methods
  • Carrier State / diagnosis
  • Carrier State / microbiology
  • Chickens / microbiology*
  • Molecular Diagnostic Techniques / methods
  • Multiplex Polymerase Chain Reaction / methods*
  • Poultry Diseases / diagnosis*
  • Poultry Diseases / microbiology
  • Salmonella / classification*
  • Salmonella / isolation & purification*
  • Salmonella Infections, Animal / diagnosis*
  • Salmonella Infections, Animal / microbiology
  • Serogroup*
  • Veterinary Medicine / methods