Hybridoma technology is a remarkable and indispensable tool for generating high-quality monoclonal antibodies. Hybridoma-derived monoclonal antibodies not only serve as powerful research and diagnostic reagents, but have also emerged as the most rapidly expanding class of therapeutic biologicals. In this chapter, an overview of hybridoma technology and the laboratory procedures used routinely for hybridoma production and antibody screening are presented, including characterization of peptide antibodies.
Keywords: Enzyme-linked immunosorbent assay; Fusion; HybER; Hybridoma enhancing reagent; Immunization; Isotype determination; Monoclonal antibody; Peptides; Screening; Selection.