Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Thouraya Chérif; Mabrouka Saidani; Dominique Decré; Ilhem Boutiba-Ben Boubaker; Guillaume Arlet

doi:10.1128/AAC.00828-15

Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia

Antimicrob Agents Chemother. 2015 Oct 12;60(1):44-51. doi: 10.1128/AAC.00828-15. Print 2016 Jan.

Authors

Thouraya Chérif¹, Mabrouka Saidani², Dominique Decré³, Ilhem Boutiba-Ben Boubaker², Guillaume Arlet⁴

Affiliations

¹ Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Tunis, Tunisie.
² Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Tunis, Tunisie CHU Charles Nicolle, Service de Microbiologie, Tunis, Tunisia.
³ Sorbonne Université, UPMC Université Paris 06 CR7, Paris, France INSERM U1135, CIMI, Team E13, Paris, France AP-HP, Groupe Hospitalier des Hôpitaux Universitaires de l'Est Parisien, Département de Bactériologie, Paris, France.
⁴ Sorbonne Université, UPMC Université Paris 06 CR7, Paris, France INSERM U1135, CIMI, Team E13, Paris, France AP-HP, Groupe Hospitalier des Hôpitaux Universitaires de l'Est Parisien, Département de Bactériologie, Paris, France guillaume.arlet@tnn.aphp.fr.

Abstract

Over a period of 40 months, plasmid-mediated AmpC β-lactamases were detected in Tunis, Tunisia, in 78 isolates (0.59%) of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. In 67 isolates, only one ampC gene was detected, i.e., blaCMY-2-type (n = 33), blaACC (n = 23), blaDHA (n = 6) or blaEBC (n = 5). Multiple ampC genes were detected in 11 isolates, with the following distribution: blaMOX-2, blaFOX-3, and blaCMY-4/16 (n = 6), blaFOX-3 and blaMOX-2 (n = 3), and blaCMY-4 and blaMOX-2 (n = 2). A great variety of plasmids carrying these genes was found, independently of the species and the bla gene. If the genetic context of blaCMY-2-type is variable, that of blaMOX-2, reported in part previously, is unique and that of blaFOX-3 is unique and new.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aminoglycosides / pharmacology
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Base Sequence
Drug Resistance, Bacterial / genetics
Enterobacteriaceae Infections / epidemiology
Enterobacteriaceae Infections / microbiology
Escherichia coli / drug effects
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Gene Expression
Humans
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Molecular Sequence Data
Penicillins / pharmacology
Plasmids / chemistry*
Plasmids / metabolism
Proteus mirabilis / drug effects
Proteus mirabilis / enzymology
Proteus mirabilis / genetics*
Proteus mirabilis / isolation & purification
Tunisia / epidemiology
Virulence Factors / genetics*
Virulence Factors / metabolism
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Aminoglycosides
Anti-Bacterial Agents
Bacterial Proteins
Penicillins
Virulence Factors
AmpC beta-lactamases
beta-Lactamases