Abstract
Bioluminescent method for measurements of the neutrophilic NAD(P)-dependent dehydrogenases (lactate dehydrogenase, NAD-dependent malate dehydrogenase, NADP-dependent decarboxylating malate dehydrogenase, NAD-dependent isocitrate dehydrogenase, and glucose- 6-phosphate dehydrogenase) is developed. The sensitivity of the method allows minimization of the volume of biological material for measurements to 104 neutrophils per analysis. The method is tried in patients with diffuse purulent peritonitis. Low levels of NADPH synthesis enzymes and high levels of enzymes determining the substrate flow by the Krebs cycle found in these patients can lead to attenuation of functional activity of cells.
Keywords:
dehydrogenases; enzyme activities; metabolism; neutrophilic granulocytes; peritonitis.
MeSH terms
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Biological Assay / standards*
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FMN Reductase / chemistry
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Glucosephosphate Dehydrogenase / metabolism*
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Humans
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Hydrogen-Ion Concentration
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Isocitrate Dehydrogenase / metabolism*
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L-Lactate Dehydrogenase / metabolism*
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Luciferases, Bacterial / chemistry
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Luminescent Measurements
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Malate Dehydrogenase (NADP+) / metabolism*
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Malate Dehydrogenase / metabolism*
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Neutrophils / chemistry
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Neutrophils / enzymology
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Neutrophils / pathology
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Peritonitis / blood
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Peritonitis / diagnosis*
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Peritonitis / pathology
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Photobacterium / chemistry
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Photobacterium / enzymology
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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L-Lactate Dehydrogenase
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Malate Dehydrogenase
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Isocitrate Dehydrogenase
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Glucosephosphate Dehydrogenase
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Malate Dehydrogenase (NADP+)
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Luciferases, Bacterial
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FMN Reductase