Hapten-Binding Bispecific Antibodies for the Targeted Delivery of SiRNA and SiRNA-Containing Nanoparticles

Methods Mol Biol. 2016:1364:219-34. doi: 10.1007/978-1-4939-3112-5_18.

Abstract

Hapten-binding bispecific antibodies (bsAbs) are effective and versatile tools for targeting diverse payloads, including siRNAs, to specific cells and tissues. In this chapter, we provide examples for successful SiRNA delivery using this powerful targeting platform. We further provide protocols for designing and producing bsAbs, for combining bsAbs with SiRNA into functional complexes, and achieving specific mRNA knockdown in cells by using these functional complexes.

Keywords: Bispecific antibodies; Dynamic polyconjugates; Fab; Hapten; IgG; Lipid nanoparticles; Recombinant antibody expression; SiRNA delivery; Targeted delivery; scFv.

MeSH terms

  • Antibodies, Bispecific / genetics
  • Antibodies, Bispecific / immunology*
  • Antibodies, Bispecific / metabolism*
  • Cytoplasm / metabolism
  • Drug Carriers / metabolism*
  • Gene Knockdown Techniques
  • Genetic Vectors / genetics
  • Haptens / immunology*
  • Humans
  • MCF-7 Cells
  • Microscopy, Fluorescence
  • Nanoparticles / chemistry*
  • Polymerase Chain Reaction
  • RNA, Small Interfering / chemistry*
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism*

Substances

  • Antibodies, Bispecific
  • Drug Carriers
  • Haptens
  • RNA, Small Interfering