Infraspecific variation within and across complete organellar genomes and nuclear ribosomal repeats in a moss

Lily R Lewis; Yang Liu; Ricardo Rozzi; Bernard Goffinet

doi:10.1016/j.ympev.2015.12.005

Infraspecific variation within and across complete organellar genomes and nuclear ribosomal repeats in a moss

Mol Phylogenet Evol. 2016 Mar:96:195-199. doi: 10.1016/j.ympev.2015.12.005. Epub 2015 Dec 24.

Authors

Lily R Lewis¹, Yang Liu², Ricardo Rozzi³, Bernard Goffinet⁴

Affiliations

¹ Department of Ecology and Evolutionary Biology, University of Connecticut, 75 North Eagleville Rd., Storrs, CT 06269, USA; Omora Ethnobotanical Park, Institute of Ecology and Biodiversity, and Universidad de Magallanes, Puerto Williams, Antarctic Province, Chile. Electronic address: LilyRLewis@gmail.com.
² Department of Ecology and Evolutionary Biology, University of Connecticut, 75 North Eagleville Rd., Storrs, CT 06269, USA. Electronic address: yang.liu@uconn.edu.
³ Department of Philosophy, University of North Texas, 1704 West Mulberry, Denton, TX 76201, USA; Omora Ethnobotanical Park, Institute of Ecology and Biodiversity, and Universidad de Magallanes, Puerto Williams, Antarctic Province, Chile. Electronic address: ricardo.rozzi@unt.edu.
⁴ Department of Ecology and Evolutionary Biology, University of Connecticut, 75 North Eagleville Rd., Storrs, CT 06269, USA. Electronic address: bernard.goffinet@uconn.edu.

PMID: 26724407
DOI: 10.1016/j.ympev.2015.12.005

Abstract

Bryophytes (mosses, liverworts, and hornworts) are diverse and ecologically and evolutionarily significant yet genome scale data sets and analyses remain extremely sparse relative to other groups of plants, and are completely lacking at the infraspecific level. By sequencing the complete organellar genomes and nuclear ribosomal repeat from seven patches of a South American sub-Antarctic neo-endemic non-model moss, we present the first characterization of infraspecific polymorphism within and across the three genomic compartments for a bryophyte. Diversity within patches is accounted for by both intraindividual and interindividual variation for the nuclear ribosomal repeat and plastid genome, respectively. This represents the most extensive infraspecific genomic dataset generated for an early land plant lineage thus far and provides insight into relative rates of substitution between organellar genomes, including high rates of nonsynonymous to synonymous substitutions.

Keywords: Bryophyte; Early land plant; Genomics; Infraspecific polymorphism; Tetraplodon.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bryophyta / cytology*
Bryophyta / genetics*
Cell Nucleus / genetics*
DNA, Ribosomal / genetics*
Datasets as Topic
Evolution, Molecular
Genome, Plant / genetics*
Genome, Plastid / genetics
Genomics*
Organelles / genetics*
Plastids / genetics
Polymorphism, Genetic

Substances

DNA, Ribosomal