Highly Fluorescent and Photostable Polymeric Nanofibers as Scaffolds for Cell Interfacing and Long-Term Tracking

Adv Healthc Mater. 2016 Mar 9;5(5):529-33. doi: 10.1002/adhm.201500693. Epub 2016 Jan 15.

Abstract

Highly fluorescent polymeric nanofibers fabricated via electrospinning of PCL-DPP-PCL (photostable polycaprolactones-di(thiophene-2-yl)-diketopyrrolopyrrole-photostable polycaprolactones) and commercial PCL mixture show superior photostability and cytocompatibility for long-term tracking of cell-substrate interaction. As a proof of concept, these PCL-DPP-PCL nanofibers enable clear visualization of intricate cell-substrate interactions such as oligodendrocyte myelination.

Keywords: electrospinning; fluorescence; myelination; oligodendrocytes; polycaprolactone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Animals
  • Cell Tracking / methods*
  • Fibroblasts / metabolism
  • Fluorescence
  • Humans
  • Light*
  • Nanofibers / chemistry*
  • Nanofibers / ultrastructure
  • Oligodendroglia / cytology
  • Polymers / chemistry*
  • Rats
  • Tissue Scaffolds / chemistry*

Substances

  • Polymers