Photoactivatable CO release from engineered protein crystals to modulate NF-κB activation

Hiroyasu Tabe; Takuya Shimoi; Marion Boudes; Satoshi Abe; Fasséli Coulibaly; Susumu Kitagawa; Hajime Mori; Takafumi Ueno

doi:10.1039/c5cc10440h

Photoactivatable CO release from engineered protein crystals to modulate NF-κB activation

Chem Commun (Camb). 2016 Mar 25;52(24):4545-8. doi: 10.1039/c5cc10440h.

Authors

Hiroyasu Tabe¹, Takuya Shimoi², Marion Boudes³, Satoshi Abe², Fasséli Coulibaly³, Susumu Kitagawa¹, Hajime Mori⁴, Takafumi Ueno²

Affiliations

¹ Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. kitagawa@icems.kyoto-u.ac.jp.
² Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 224-8501, Japan. tueno@bio.titech.ac.jp.
³ Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC3800, Australia.
⁴ Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

PMID: 26940021
DOI: 10.1039/c5cc10440h

Abstract

Photoactivatable CO releasing protein crystals were developed by immobilization of Mn carbonyl complexes in polyhedral crystals, which are spontaneously formed in insect cells. The photoactivatable CO release from the engineered protein crystals activates nuclear factor kappa B (NF-κB) upon stimulation by visible light irradiation with suppression of cytotoxicity of the Mn complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon Monoxide / chemistry*
NF-kappa B / metabolism*
Proteins / chemistry*
Spodoptera

Substances

NF-kappa B
Proteins
Carbon Monoxide