Anti-tumor and anti-virus activity of polysaccharides extracted from Sipunculus nudus(SNP) on Hepg2.2.15

Jie Su; Linlin Jiang; Jingna Wu; Zhiyu Liu; Yuping Wu

doi:10.1016/j.ijbiomac.2016.03.022

Anti-tumor and anti-virus activity of polysaccharides extracted from Sipunculus nudus(SNP) on Hepg2.2.15

Int J Biol Macromol. 2016 Jun:87:597-602. doi: 10.1016/j.ijbiomac.2016.03.022. Epub 2016 Mar 14.

Authors

Jie Su¹, Linlin Jiang², Jingna Wu², Zhiyu Liu³, Yuping Wu⁴

Affiliations

¹ South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 519000, PR China; Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fisheries Research Institute of Fujian, Xiamen, Fujian 361012, PR China.
² Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fisheries Research Institute of Fujian, Xiamen, Fujian 361012, PR China.
³ Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fisheries Research Institute of Fujian, Xiamen, Fujian 361012, PR China. Electronic address: 272132622@qq.com.
⁴ South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 519000, PR China. Electronic address: exwyp@163.com.

PMID: 26987430
DOI: 10.1016/j.ijbiomac.2016.03.022

Abstract

Many polysaccharides have biological activities and have been investigated for their antitumor effects. In this study, we investigated the anti-tumor activity and anti-virus activity of SNP-the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15. Flow cytometry analysis demonstrated that SNP induced dose-dependent cell apoptosis on Hepg2.2.15. Real-time PCR and Western Blot analysis showed that SNP down-regulated the synthesis of HBsAg, HBV-DNA and enhanced the expression of pro-apoptosis proteins TNF-α, caspase-3, and Bax, while decreasing the expression of the anti-apoptosis proteins survivin, Bcl-2, and VEGF. These results suggested that SNP suppressed cell viability of Hepg2.2.15 and that could be a novel anti-tumor and anti-HBV agent.

Keywords: Anti-tumor activity; Anti-virus activity; Polysaccharide; Sipunculus nudus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / pharmacology*
Antiviral Agents / chemistry*
Antiviral Agents / isolation & purification
Antiviral Agents / pharmacology*
Apoptosis / drug effects
Aquatic Organisms / chemistry*
Caspase 3 / metabolism
Cell Cycle Checkpoints / drug effects
DNA, Viral / genetics
Gene Expression Regulation, Viral / drug effects
Hep G2 Cells
Hepatitis B Surface Antigens / biosynthesis
Hepatitis B virus / drug effects
Hepatitis B virus / genetics
Hepatitis B virus / metabolism
Humans
Inhibitor of Apoptosis Proteins / metabolism
Polysaccharides / chemistry
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Survivin
Tumor Necrosis Factor-alpha / metabolism
Vascular Endothelial Growth Factor A / metabolism
bcl-2-Associated X Protein / metabolism

Substances

Antineoplastic Agents
Antiviral Agents
BIRC5 protein, human
DNA, Viral
Hepatitis B Surface Antigens
Inhibitor of Apoptosis Proteins
Polysaccharides
Proto-Oncogene Proteins c-bcl-2
Survivin
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A
bcl-2-Associated X Protein
Caspase 3