1 State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China. genxili@nju.edu.cn.
2 Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P. R. China.
3 State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China. genxili@nju.edu.cn and Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, P. R. China.
A novel colourimetric method for protein assays is proposed based on proximity ligation induced assembly of Mg(2+)-dependent DNAzymes, which may offer simple, cost-effective, sensitive and selective detection of the target protein.