Rapid and sensitive fluorescent imaging of tiny tumors in vivo and in clinical specimens

Mako Kamiya; Yasuteru Urano

doi:10.1016/j.cbpa.2016.04.004

Rapid and sensitive fluorescent imaging of tiny tumors in vivo and in clinical specimens

Curr Opin Chem Biol. 2016 Aug:33:9-15. doi: 10.1016/j.cbpa.2016.04.004. Epub 2016 Apr 19.

Authors

Mako Kamiya¹, Yasuteru Urano²

Affiliations

¹ Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
² Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; CREST, Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan. Electronic address: uranokun@m.u-tokyo.ac.jp.

PMID: 27100047
DOI: 10.1016/j.cbpa.2016.04.004

Abstract

Fluorescence-guided diagnostics is one of the most powerful techniques for real-time in situ tumor detection. Here, we introduce two categories of fluorescence probes used for tumor imaging (always-on probes and activatable probes) and briefly summarize recent advances in tumor-targeted fluorescence imaging probes and their clinical/preclinical applications, including our recent work on rational design of activatable fluorescence probes for tumors expressing aminopeptidases and glycosidases. These probes enable rapid and sensitive detection of tiny tumors as small as <1mm in diameter, both in vivo and in clinical specimens.

Publication types

Review

MeSH terms

Humans
Neoplasms / diagnostic imaging*
Optical Imaging / methods*
Sensitivity and Specificity