Querectin Alleviates Zinc Oxide Nanoreprotoxicity in Male Albino Rats

Mohamed M A Hussein; Haytham A Ali; Islam M Saadeldin; Mona M Ahmed

doi:10.1002/jbt.21812

Querectin Alleviates Zinc Oxide Nanoreprotoxicity in Male Albino Rats

J Biochem Mol Toxicol. 2016 Oct;30(10):489-496. doi: 10.1002/jbt.21812. Epub 2016 Apr 25.

Authors

Mohamed M A Hussein¹, Haytham A Ali², Islam M Saadeldin^{3

4}, Mona M Ahmed⁵

Affiliations

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt. hamza_vet@yahoo.com.
² Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.
³ Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.
⁴ Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, KSA.
⁵ Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.

PMID: 27111109
DOI: 10.1002/jbt.21812

Abstract

Zinc oxide nanopartciles (ZnONPs) involved in advanced technologies, and their wide-scale use in consumer market makes human beings more prone to the exposure to ZnONPs. The present study was undertaken to evaluate amelioration of ZnONP-induced toxicities with querectin in male albino rats. ZnONPs-treated rats showed a significant decrease in sperm cell count, sperm motility, live and normal sperms, as well as serum testosterone level. Severe histopathological damage with a significant increase in lipid peroxidation and a decrease in antioxidant enzymes activity and the GSH level were observed in the affected testis. Relative quantitative polymerase chain reaction results showed a significant decrease in antioxidant enzymes (superoxide dismutase and catalase) and a significant decrease in 3β-HSD, 17β-HSD, and Nr5A1 transcripts. Rats-administered querectin along with ZnONPs showed less toxic effects on all studied reproductive traits and mRNA transcripts. Our results suggest that querectin is beneficial for preventing or ameliorating ZnONP reproductive toxicities in males.

Keywords: Antioxidants; Male Rats; Querectin; Sperm; Zinc Oxide Nanoparticles.

MeSH terms

17-Hydroxysteroid Dehydrogenases / genetics
17-Hydroxysteroid Dehydrogenases / metabolism
3-Hydroxysteroid Dehydrogenases / genetics
3-Hydroxysteroid Dehydrogenases / metabolism
Administration, Oral
Animals
Antioxidants / pharmacology*
Catalase / genetics
Catalase / metabolism
Gene Expression Regulation
Glutathione Peroxidase / genetics
Glutathione Peroxidase / metabolism
Lipid Peroxidation / drug effects
Male
Metal Nanoparticles / toxicity*
Oxidative Stress / drug effects
Quercetin / pharmacology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Sperm Count
Sperm Motility / drug effects
Spermatozoa / cytology
Spermatozoa / drug effects*
Spermatozoa / metabolism
Steroidogenic Factor 1 / genetics
Steroidogenic Factor 1 / metabolism
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism
Testis / cytology
Testis / drug effects*
Testis / metabolism
Testosterone / blood
Zinc Oxide / antagonists & inhibitors
Zinc Oxide / toxicity*

Substances

Antioxidants
RNA, Messenger
Steroidogenic Factor 1
steroidogenic factor 1, rat
Testosterone
Quercetin
17-Hydroxysteroid Dehydrogenases
3-Hydroxysteroid Dehydrogenases
Catalase
Glutathione Peroxidase
Superoxide Dismutase
Zinc Oxide